Conferencia Dr. Pereira Lima

Implementation of entomological surveillance to direct actions against Aedes (Diptera, Culicidae) in Brazil

Lima, J B P¹, Lemos, P S², Bellinato, D F¹, Alves L D³, Py-Daniel, S S², Frutuoso L C V², Passos R A².

¹Laboratório de Biologia, Controle e Vigilância de Insetos Vetores – LBCVIV, Instituto Oswaldo Cruz – IOC, Fundação Oswaldo Cruz – Fiocruz, Brazil.
²Coordenação-Geral de Vigilância de Arboviroses – CGARB, Departamento de Doenças Transmissíveis – DEDT, Secretaria de Vigilância em Saúde e Ambiente – SVSA, Ministério da Saúde – MS, Brazil.
³Centro Federal de Educação Tecnológica Celso Suckow da Fonseca (Cefet/RJ), Brazil.

In 2022, the Ministry of Health launched a strategy to strengthen entomological surveillance by using ovitraps to direct Aedes mosquito control efforts in Brazil. The Egg Count tool, which monitors vector populations based on egg density and generates heat maps for strategic targeting of control efforts, has also begun to be used. To describe the expansion of the use of ovitraps and entomological risk maps in decision-making for Aedes control in Brazilian municipalities. We analysed the implementation of ovitraps in municipalities between 2022 and 2025, based on workshops held in the states and municipalities. During the workshops, municipalities received guidance on the installation and georeferencing of ovitraps, the timing of pallet replacement, reading eggs deposited on the pallets, and entering data into the Egg Count app, which calculates infestation indexes and produces maps indicating the most critical areas. In 2022, we launched the project with 22 municipalities in RS. In 2023, we expanded to the states of BA, CE, DF, GO, MS, MG, PR, RJ, and TO, totalling 139 locations, a 532% increase over the previous year. In 2024, we expanded to 402 locations, including the aforementioned states, as well as incorporating AP, AM, ES, and PA, representing a 189% increase over 2023. In 2025, there was a notable expansion to the states of MT, PI, SC, and some indigenous territories in MS, RS, BA, and PE, reaching 589 locations —a 46% increase compared to 2024. 120,000 traps were distributed, and of these, 53,193 are installed (44%), which enabled the removal of 24.3 million eggs from the environment and directed environmental management actions, mechanical control, and the application of larvicides to the areas of highest infestation. The municipalities reported a reduction in vector density after implementing targeted actions, which highlights the relevance of using this methodology to direct control actions. The increased use of ovitraps and maps to direct actions strengthens integrated vector management in municipalities, providing more efficient and targeted control. In 2025, ovitraps were recommended in the National Guidelines for the Prevention and Control of Urban Arboviruses as an entomological surveillance strategy for directing actions and monitoring new vector control technologies. The participation of states and municipalities was crucial to the implementation and expansion of the strategy in Brazil.

Keywords: Aedes, oviposition traps, vector surveillance, control.